Format

Send to

Choose Destination
Nanomedicine (Lond). 2012 Nov;7(11):1657-66. doi: 10.2217/nnm.12.21. Epub 2012 May 14.

Modification of plasmid DNA topology by 'histone-mimetic' gold nanoparticles.

Author information

1
Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Mariano Esquillor s/n 50018, Zaragoza, Spain.

Abstract

AIMS:

Our aim is to explore whether gold nanoparticles (AuNPs) functionalized with a carboxylated polyethylene glycol (PEG) and protamine (AuNP@PEG@Prot) can modulate - enhance or restrain - DNA condensation, altering DNA conformation and inducing structural changes. Understanding how these nanoconjugates modulate DNA structure, size and shape of DNA condensates, and enable control over the resulting 3D structures is of major biological and therapeutic importance.

MATERIALS & METHODS:

Citrate-AuNPs were covered with a dense layer of a hetero-functional octa(ethylene glycol) (SH-EG(8)-COOH). Conjugation of protamine to the AuNP@PEG was achieved by taking advantage of the carboxylated surface previously generated on the surface of the NP and the remaining amino groups from the protamine, using carbodiimide and N-hydroxysulfosuccinimide coupling reactions.

RESULTS & CONCLUSION:

AuNP@PEG@Prot modulates the structure and topology of DNA, not only for condensation, but also for decondensation, via formation of higher quantities of dimers and multimers, when compared with AuNP@PEG and free protamine.

PMID:
22583575
DOI:
10.2217/nnm.12.21
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center