Format

Send to

Choose Destination
J Microsc. 2012 Jun;246(3):213-20. doi: 10.1111/j.1365-2818.2012.03600.x. Epub 2012 Apr 12.

Microscopy beyond the diffraction limit using actively controlled single molecules.

Author information

1
Department of Chemistry, Stanford University, Stanford, California 94305, USA. wmoerner@stanford.edu

Abstract

In this short review, the general principles are described for obtaining microscopic images with resolution beyond the optical diffraction limit with single molecules. Although it has been known for several decades that single-molecule emitters can blink or turn on and off, in recent work the addition of on/off control of molecular emission to maintain concentrations at very low levels in each imaging frame combined with sequential imaging of sparse subsets has enabled the reconstruction of images with resolution far below the optical diffraction limit. Single-molecule active control microscopy provides a powerful window into information about nanoscale structures that was previously unavailable.

PMID:
22582796
PMCID:
PMC3727413
DOI:
10.1111/j.1365-2818.2012.03600.x
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center