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Guang Pu Xue Yu Guang Pu Fen Xi. 2012 Mar;32(3):630-4.

[FTIR analysis of the impact of covalent coupling on the secondary structure of antibody protein].

[Article in Chinese]

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College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.


The immunolatex was prepared by covalent coupling. FTIR technology combined with substractive spectroscopy, deconvolution, derivation and curve-fitting methods were used to study the structure of the antibody protein on the immunolatex. The result demonstrates that the alpha-helix strcture of antibody increases with the increase in the pH value and the concentration of latex. So it is concluded that covalent coupling has a great impact on the secondary structure of antibody protein.

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