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Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021.

Rational design of true monomeric and bright photoactivatable fluorescent proteins.

Author information

1
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Abstract

Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations and forms aggregates when labeling membrane proteins, limiting its application as a fusion partner. We solved the crystal structure of tetrameric mEos2 and rationally designed improved versions, mEos3.1 and mEos3.2, that are truly monomeric, are brighter, mature faster and exhibit higher photon budget and label density.

PMID:
22581370
DOI:
10.1038/nmeth.2021
[Indexed for MEDLINE]

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