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Methods Mol Biol. 2012;876:207-16. doi: 10.1007/978-1-61779-809-2_17.

Genome-wide profiling of uncapped mRNA.

Author information

1
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang, Beijing, China. jiao@caltech.edu

Abstract

Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRNA synthesis and degradation. In this chapter, we describe a method to profile these uncapped mRNAs at the genome scale.

PMID:
22576098
DOI:
10.1007/978-1-61779-809-2_17
[Indexed for MEDLINE]

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