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Biomed Opt Express. 2012 Apr 1;3(4):735-40. doi: 10.1364/BOE.3.000735. Epub 2012 Mar 15.

Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography.

Abstract

Micro-scale, non-invasive, three-dimensional cross-sectional imaging of protein crystals was successfully accomplished using ultra-high resolution optical coherence tomography (UHR-OCT) with low noise, Gaussian like supercontinuum. This technique facilitated visualization of protein crystals even those in medium that also contained substantial amounts of precipitates. We found the enhancement of the scattered signal from protein crystal by inclusion of agarose gel in the crystallization medium. Crystals of a protein and a salt in the same sample when visualized by UHR-OCT showed distinct physical characteristics, suggesting that protein and salt crystals may, in general, be distinguishable by UHR-OCT. UHR-OCT is a nondestructive and rapid method, which should therefore find use in automated systems designed to visualize crystals.

KEYWORDS:

(110.4500) Optical coherence tomography; (170.3880) Medical and biological imaging

PMID:
22574261
PMCID:
PMC3345802
DOI:
10.1364/BOE.3.000735
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