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J Microbiol Immunol Infect. 2012 Jun;45(3):185-92. doi: 10.1016/j.jmii.2011.11.004. Epub 2012 May 8.

Molecular detection and incidence of human papillomavirus in neonates: methodology and a pilot study in a medical center.

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1
Department of Pediatrics, National Taiwan University Hospital, College of Medicine, Taipei, Taiwan.

Abstract

BACKGROUND/PURPOSE:

Human papillomavirus (HPV) infection can cause laryngeal papillomas in children. Vertical transmission has been confirmed. This study aimed to establish a sensitive molecular diagnostic method and understand the incidence of the HPV-6 and HPV-11 in neonates with intubation.

METHODS:

We enrolled 108 newborns between October 2007 and January 2010. All neonates were intubated due to underlying disease. The specimens were collected via endotracheal aspiration. DNA of HPV types 6 and 11 was detected by real-time PCR and nested PCR.

RESULTS:

HPV-DNA was detected in eight of the 108 newborns studied. Seven respiratory specimens tested positive for HPV-11 and one was positive for HPV-6. The HPV 6/11 detection rate in neonates was 7.4% (8/108).

CONCLUSION:

A rapid, sensitive, specific, and reproducible RT-PCR method and nest PCR were developed for the detection and differentiation of HPV-6 and HPV-11 genomic variants in a single PCR reaction. The assays are of great value for clinical and epidemiologic studies of HPV-6 and HPV-11 infections. Neonatal HPV colonization may be related to juvenile-onset recurrent respiratory papillomatosis. The transmission route may be from mother to child. The clinical significance of neonatal carriage of HPV-6 or HPV-11 warrants further study.

PMID:
22571996
DOI:
10.1016/j.jmii.2011.11.004
[Indexed for MEDLINE]
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