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PLoS Comput Biol. 2012;8(4):e1002467. doi: 10.1371/journal.pcbi.1002467. Epub 2012 Apr 26.

Deconvolution of the cellular force-generating subsystems that govern cytokinesis furrow ingression.

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1
Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America.

Abstract

Cytokinesis occurs through the coordinated action of several biochemically-mediated stresses acting on the cytoskeleton. Here, we develop a computational model of cellular mechanics, and using a large number of experimentally measured biophysical parameters, we simulate cell division under a number of different scenarios. We demonstrate that traction-mediated protrusive forces or contractile forces due to myosin II are sufficient to initiate furrow ingression. Furthermore, we show that passive forces due to the cell's cortical tension and surface curvature allow the furrow to complete ingression. We compare quantitatively the furrow thinning trajectories obtained from simulation with those observed experimentally in both wild-type and myosin II null Dictyostelium cells. Our simulations highlight the relative contributions of different biomechanical subsystems to cell shape progression during cell division.

PMID:
22570593
PMCID:
PMC3343096
DOI:
10.1371/journal.pcbi.1002467
[Indexed for MEDLINE]
Free PMC Article
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