Genetic incorporation of D-lysine into diketoreductase in Escherichia coli cells

Amino Acids. 2012 Dec;43(6):2553-9. doi: 10.1007/s00726-012-1311-1. Epub 2012 May 9.

Abstract

Pyrococcus horikoshii lysyl-tRNA synthetase/tRNA orthogonal pair exhibited high selectivity towards D-lysine in the presence of excess amount of D-lysine. Based on the observation, this orthogonal pair was employed to encode D-lysine, and D-lysine was site-specifically incorporated into the diketoreductase in E. coli cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / cytology
  • Escherichia coli / enzymology*
  • Escherichia coli / metabolism
  • Lysine / genetics*
  • Models, Molecular
  • Oxidoreductases / genetics*
  • Oxidoreductases / metabolism

Substances

  • Oxidoreductases
  • Lysine