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Biochem Biophys Res Commun. 1990 Nov 30;173(1):351-7.

Purification and identification of creatine phosphokinase B as a substrate of protein kinase C in mouse skin in vivo.

Author information

1
Department of Cancer Cell Research, University of Tokyo, Japan.

Abstract

We previously described epidermal proteins with molecular weights of 40,000 (p40) and 34,000 (p34) as target proteins of protein kinase C in mouse skin carcinogenesis in vivo. In the present work, p40 was purified from mouse brain by the use of 32P-labeled p40 of BALB/MK-2 cells as a tracer. Following four lines of evidence indicate that p40 is creatine phosphokinase B. 1) The amino acid sequences of all peptide fragments of p40 from mouse brain were located in the primary structure of creatine phosphokinase B. 2) p40 of BALB/MK-2 cells was immunoprecipitated with goat antibody against human creatine phosphokinase B. 3) p40 of BALB/MK-2 cells was absorbed to and eluted from a creatine affinity column. 4) Purified creatine phosphokinase B was phosphorylated in vitro by purified protein kinase C, but not by cAMP-dependent kinase or casein kinase II.

PMID:
2256926
DOI:
10.1016/s0006-291x(05)81064-2
[Indexed for MEDLINE]

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