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FEBS Lett. 2012 Apr 5;586(7):1004-8. doi: 10.1016/j.febslet.2012.02.028. Epub 2012 Mar 6.

Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.

Author information

1
Department of Biology, California State University-Fresno, Fresno, CA 93740, USA.

Abstract

The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.

PMID:
22569254
PMCID:
PMC3668096
DOI:
10.1016/j.febslet.2012.02.028
[Indexed for MEDLINE]
Free PMC Article

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