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FEBS Lett. 2012 Apr 5;586(7):977-83. doi: 10.1016/j.febslet.2012.02.052. Epub 2012 Mar 8.

Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.

Author information

1
Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

Abstract

RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of RBM5 is indispensable for its ability to interact with DHX15. Strikingly, we find that RBM5 stimulates the helicase activity of DHX15 in a G patch domain-dependent manner in vitro. Helicase activities play critical roles in modulating pre-mRNA splicing. Our findings thus suggest a new mechanism underlying the regulatory roles of RBM5 in pre-mRNA splicing.

PMID:
22569250
DOI:
10.1016/j.febslet.2012.02.052
[Indexed for MEDLINE]
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