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PLoS One. 2012;7(5):e36442. doi: 10.1371/journal.pone.0036442. Epub 2012 May 2.

Biased gene fractionation and dominant gene expression among the subgenomes of Brassica rapa.

Author information

1
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

Abstract

Polyploidization, both ancient and recent, is frequent among plants. A "two-step theory" was proposed to explain the meso-triplication of the Brassica "A" genome: Brassica rapa. By accurately partitioning of this genome, we observed that genes in the less fractioned subgenome (LF) were dominantly expressed over the genes in more fractioned subgenomes (MFs: MF1 and MF2), while the genes in MF1 were slightly dominantly expressed over the genes in MF2. The results indicated that the dominantly expressed genes tended to be resistant against gene fractionation. By re-sequencing two B. rapa accessions: a vegetable turnip (VT117) and a Rapid Cycling line (L144), we found that genes in LF had less non-synonymous or frameshift mutations than genes in MFs; however mutation rates were not significantly different between MF1 and MF2. The differences in gene expression patterns and on-going gene death among the three subgenomes suggest that "two-step" genome triplication and differential subgenome methylation played important roles in the genome evolution of B. rapa.

PMID:
22567157
PMCID:
PMC3342247
DOI:
10.1371/journal.pone.0036442
[Indexed for MEDLINE]
Free PMC Article

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