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Methods Mol Biol. 2012;887:95-110. doi: 10.1007/978-1-61779-860-3_10.

Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs.

Author information

1
Department of Oral Biology, University of Oslo, Oslo, Norway. harald.osmundsen@odont.uio.no

Abstract

The use of deoxyoligonucleotide microarrays facilitates rapid expression profiling of gene expression using samples of about 1 μg of total RNA. Here are described practical aspects of the procedures involved, including essential reagents. Analysis of results is discussed from a practical, experimental, point of view together with software required to carry out the required statistical analysis to isolate populations of differentially expressed genes.

PMID:
22566050
DOI:
10.1007/978-1-61779-860-3_10
[Indexed for MEDLINE]

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