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J Forensic Sci. 2012 Nov;57(6):1570-3. doi: 10.1111/j.1556-4029.2012.02174.x. Epub 2012 May 4.

Specificity, sensitivity, and operability of RSID™-urine for forensic identification of urine: comparison with ELISA for Tamm-Horsfall protein.

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1
Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, Kashiwa, Chiba, Japan. tomoko@nrips.go.jp

Abstract

In this study, the specificity, sensitivity, and operability of RSID™-Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm-Horsfall protein (THP). Urine was successfully identified among other body fluids using RSID™-Urine and ELISA detection of THP. The detection limit of RSID™-Urine equated to 0.5 μL of urine; although the sensitivity of RSID™-Urine may be lower than that of ELISA detection of THP, it is thought to be sufficient for application to casework samples. However, results from RSID™-Urine must be interpreted with caution when the sample may have been contaminated with blood or vaginal fluid, because this might inhibit urine detection. The RSID™-Urine assay can be performed in just 15 min by dropping the extracted sample onto the test cassette. Therefore, RSID™-Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP.

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