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Beilstein J Org Chem. 2012;8:640-9. doi: 10.3762/bjoc.8.71. Epub 2012 Apr 25.

Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique.

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Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.


We screened a randomized library and identified natural peptides that bound selectively to a chimeric peptide containing α-, β- and γ-amino acids. The SPOT arrays provide a means for the systematic study of the possible interaction space accessible to the αβγ-chimera. The mutational analysis reveals the dependence of the binding affinities of α-peptides to the αβγ-chimera, on the hydrophobicity and bulkiness of the side chains at the corresponding hydrophobic interface. The stability of the resulting heteroassemblies was further confirmed in solution by CD and thermal denaturation.


SPOT technique; coiled coil; foldamer; screening libraries; β- and γ-amino acids

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