Format

Send to

Choose Destination
Nat Cell Biol. 2012 May 6;14(6):614-24. doi: 10.1038/ncb2495.

Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.

Author information

1
European Institute of Oncology, Department of Experimental Oncology, Via Adamello 16, 20139 Milan, Italy.

Erratum in

  • Nat Cell Biol. 2013 Mar;15(3):335.

Abstract

Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the Aurora B(Ipl1) and MPS1(Mps1) kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.

Comment in

PMID:
22561345
PMCID:
PMC3438452
DOI:
10.1038/ncb2495
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center