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Toxicol Lett. 2012 Jun 20;211(3):319-24. doi: 10.1016/j.toxlet.2012.04.014. Epub 2012 Apr 26.

Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. elegans model.

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1
Universidade Federal do Pampa, BR 472 Km 585, CEP 97500-970, Uruguaiana, RS, Brazil.

Abstract

Work with sub-natural levels of deuterium (D) in animals has demonstrated an anti-cancer effect of low D-concentration in water. Our objective was to investigate whether deuterium-depleted water (DDW) can overturn reverse manganese (Mn)-induced reduction in life span, using the Caenorhabditis elegans (C. elegans) as a model system. DDW per se had no effect on worm's life span 48 h after treatment; however, it reversed the Mn-induced decrease in C. elegans life span. Mn reduced DAF-16 levels, a transcription factor strongly associated with life-span regulation. Low D-concentration (90 ppm) restored the Mn-induced changes in DAF-16 to levels indistinguishable from controls, suggesting DDW can regulate the DAF-16 pathway. We further show that insulin-like receptor DAF-2 levels were unaltered by Mn exposure, tAKT levels increased, whilst superoxide dismutase (SOD-3) levels were decreased by Mn. DDW (90 ppm) restored the levels of tAKT and superoxide dismutase (SOD) to control values without changing DAF-2 levels. Treatment of Mn exposed worms with DDW (90 ppm) restored life-span, DAF-16 and SOD-3 levels to control levels, strongly suggesting that low D concentrations can protect against Mn toxic effects.

PMID:
22561170
PMCID:
PMC4917383
DOI:
10.1016/j.toxlet.2012.04.014
[Indexed for MEDLINE]
Free PMC Article

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