Ag/Au bi-metallic film based color surface plasmon resonance biosensor with enhanced sensitivity, color contrast and great linearity

Biosens Bioelectron. 2012 Jun-Jul;36(1):192-8. doi: 10.1016/j.bios.2012.04.016. Epub 2012 Apr 21.

Abstract

In wavelength surface plasmon resonance (SPR) biosensor, the manipulation of SPR dispersion relation by Ag/Au bi-metallic film was first time implemented. Due to the enhanced resonant wavelength shift and the sharper SPR slope of using Ag/Au bi-metallic film, the illuminated color of reflection shows one order of magnitude greater contrast than conventional SPR biosensors. Such an Ag/Au bi-metallic film based color SPR biosensor (CSPRB) allows the detail bio-interactions, for example 100 nM streptavidin, to be distinguished by directly observing the color change of reflection through naked eyes rather than the analysis of spectrometer. In addition to the enhanced sensitivity and color contrast, this CSPRB also possesses a great linear detection range up to 0.0254 RIU, which leading to the application of point-of-care tests.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Biotin / chemistry
  • Color
  • Glucose / analysis*
  • Gold / chemistry*
  • Sensitivity and Specificity
  • Silver / chemistry*
  • Streptavidin / chemistry
  • Surface Plasmon Resonance

Substances

  • Silver
  • Biotin
  • Gold
  • Streptavidin
  • Glucose