Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2012;7(4):e36023. doi: 10.1371/journal.pone.0036023. Epub 2012 Apr 27.

Intersectin (ITSN) family of scaffolds function as molecular hubs in protein interaction networks.

Author information

1
Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois, USA.

Abstract

Members of the intersectin (ITSN) family of scaffold proteins consist of multiple modular domains, each with distinct ligand preferences. Although ITSNs were initially implicated in the regulation of endocytosis, subsequent studies have revealed a more complex role for these scaffold proteins in regulation of additional biochemical pathways. In this study, we performed a high throughput yeast two-hybrid screen to identify additional pathways regulated by these scaffolds. Although several known ITSN binding partners were identified, we isolated more than 100 new targets for the two mammalian ITSN proteins, ITSN1 and ITSN2. We present the characterization of several of these new targets which implicate ITSNs in the regulation of the Rab and Arf GTPase pathways as well as regulation of the disrupted in schizophrenia 1 (DISC1) interactome. In addition, we demonstrate that ITSN proteins form homomeric and heteromeric complexes with each other revealing an added level of complexity in the function of these evolutionarily conserved scaffolds.

PMID:
22558309
PMCID:
PMC3338775
DOI:
10.1371/journal.pone.0036023
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center