Lack of evidence for integration of Trypanosoma cruzi minicircle DNA in South American human genomes

Olga Flegontova; Julius Lukeš; Pavel Flegontov

doi:10.1016/j.ijpara.2012.04.001

Lack of evidence for integration of Trypanosoma cruzi minicircle DNA in South American human genomes

Int J Parasitol. 2012 May 1;42(5):437-41. doi: 10.1016/j.ijpara.2012.04.001. Epub 2012 Apr 24.

Authors

Olga Flegontova¹, Julius Lukeš, Pavel Flegontov

Affiliation

¹ Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 České Budějovice (Budweis), Czech Republic.

PMID: 22549024
DOI: 10.1016/j.ijpara.2012.04.001

Abstract

Horizontal gene transfer involving kinetoplast DNA minicircles between Trypanosoma cruzi and its mammalian hosts has recently been proposed as a usual consequence of infection (Hecht et al., 2010). However, we have found no sequences longer than 29 bp perfectly matching minicircles of T. cruzi in the unassembled reads from Colombian and Peruvian human populations provided by the 1,000 Genome project (129 individuals in total, coverage from 1.4× to 36.3×, read length from 42 to 101 bp). The weak sequence matches that were identified are shared with a Finnish population used as a control from a non-endemic area.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colombia
DNA, Kinetoplast / genetics*
Genome, Human*
Humans
Peru
Recombination, Genetic*
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Trypanosoma cruzi / genetics*

Substances

DNA, Kinetoplast