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Nat Methods. 2012 Jun;9(6):588-90. doi: 10.1038/nmeth.1994. Epub 2012 Apr 29.

An optimized two-finger archive for ZFN-mediated gene targeting.

Author information

1
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, USA.

Abstract

The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.

PMID:
22543349
PMCID:
PMC3443678
DOI:
10.1038/nmeth.1994
[Indexed for MEDLINE]
Free PMC Article

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