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Nat Methods. 2012 Jun;9(6):582-4. doi: 10.1038/nmeth.1991. Epub 2012 Apr 29.

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies.

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1
Institute of Biochemistry, ETH Zurich, Switzerland.

Abstract

We developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy. By targeting GFP with small, high-affinity antibodies coupled to organic dyes, we achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells. We show that in combination with libraries encoding GFP-tagged proteins, virtually any known protein can immediately be used in super-resolution microscopy and that simplified labeling schemes allow high-throughput super-resolution imaging.

PMID:
22543348
DOI:
10.1038/nmeth.1991
[Indexed for MEDLINE]
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