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Biosens Bioelectron. 2012 Jun-Jul;36(1):56-61. doi: 10.1016/j.bios.2012.03.037. Epub 2012 Apr 6.

Label-free, multiplexed detection of bacterial tmRNA using silicon photonic microring resonators.

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1
Department of Biotechnology, Institute of Molecular and Cellular Biology, University of Tartu, Tartu, Estonia.

Abstract

A label-free biosensing method for the sensitive detection and identification of bacterial transfer-messenger RNA (tmRNA) is presented employing arrays of silicon photonic microring resonators. Species specific tmRNA molecules are targeted by complementary DNA capture probes that are covalently attached to the sensor surface. Specific hybridization is monitored in near real-time by observing the resonance wavelength shift of each individual microring. The sensitivity of the biosensing platform allowed for detection down to 53 fmol of Streptococcus pneumoniae tmRNA, equivalent to approximately 3.16×10(7) CFU of bacteria. The simplicity and scalability of this biosensing approach makes it a promising tool for the rapid identification of different bacteria via tmRNA profiling.

PMID:
22541813
PMCID:
PMC3367093
DOI:
10.1016/j.bios.2012.03.037
[Indexed for MEDLINE]
Free PMC Article
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