(A–B) Parietal cortex from mice and inferior parietal cortex from humans was dissected and VGSC levels were determined by western blot analyses. (A) Representative western blots and quantification of Nav1.1, Nav1.2, Nav1.3, and Nav1.6 in hAPPJ20 and NTG mice. (B) Representative western blots and quantification of Nav1.1, Nav1.2, and Nav1.6 levels in nondemented controls and AD cases.
(C–G) Double fluorescence in situ hybridization for Nav1.1 (red) and immunohistochemistry for PV (green) on cryosections from NTG, hAPPJ20, and GAD67eGFP mice. (C) In NTG mice, Nav1.1 mRNA was expressed at high levels by PV cells. Arrowheads in bottom panels (merged) indicate single- (PV; green, Nav1.1; red) or double-labeled (yellow) cells. (D, E) In GAD67eGFP mice, Nav1.1 (D) and Nav1.6 (E) mRNAs were highly expressed by GAD67eGFP-positive (green) cells. (F) In hAPPJ20 mice, Nav1.1 mRNA expression was reduced in the parietal cortex (compare with C). (G) hAPPJ20 mice had no reductions of PV cells in the parietal cortex (left) but had fewer Nav1.1-positive PV cells (middle) and lower Nav1.1 mRNA expression in PV cells (right) than controls. *p<0.05, **p<0.01, ***p<0.001 (t test). Scale bars: 50 µm (C, F); 5 µm (D, E). Values are mean ± SEM. Numbers in bars are mice (A), human cases (B), or cells (G). See .