Format

Send to

Choose Destination
Virology. 2012 Jul 20;429(1):21-8. doi: 10.1016/j.virol.2012.03.027. Epub 2012 Apr 24.

Human herpesvirus 6 glycoprotein M is essential for virus growth and requires glycoprotein N for its maturation.

Author information

1
Division of Clinical Virology, Kobe University Graduate School of Medicine, Chuo-ku, Kobe, Japan.

Abstract

Human herpesvirus 6 (HHV-6) is a T-lymphotropic virus belonging to the betaherpesvirus family. Several HHV-6-encoded glycoproteins are required for cell entry and virion maturation. Glycoprotein M (gM) is conserved among all herpesviruses, and therefore thought to have important functions; however, the HHV-6 g has not been characterized. Here, we examined the expression of HHV-6 g, and examined its function in viral replication, using a mutant and revertant gM. HHV-6 g was expressed on virions as a glycoprotein modified with complex N-linked oligosaccharides. As in other herpesviruses, HHV-6 g formed a complex with glycoprotein N (gN), and was transported from the endoplasmic reticulum to the trans-Golgi network only when part of this complex. Finally, a gM mutant virus in which the gM start codon was destroyed was not reconstituted, although its revertant was, indicating that HHV-6 g is essential for virus production, unlike the gM of alphaherpesviruses.

PMID:
22537811
DOI:
10.1016/j.virol.2012.03.027
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center