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Bioresour Technol. 2012 Jul;116:334-9. doi: 10.1016/j.biortech.2012.03.103. Epub 2012 Apr 5.

Efficient production of L-lactic acid with high optical purity by alkaliphilic Bacillus sp. WL-S20.

Author information

1
National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Abstract

Highly efficient polymer-grade L-lactic acid production was achieved by an alkaliphilic strain Bacillus sp. WL-S20 using inexpensive peanut meal as nitrogen source and sodium hydroxide as neutralizing agent. In multi-pulse fed-batch fermentation of Bacillus sp. WL-S20, a L-lactic acid concentration of 225 g/l with a yield of 99.3% was obtained. In single-pulse fed-batch fermentation, a concentration of 180 g/l was obtained with a yield of 98.6%. No D-isomers of lactic acid were detected. The production of a high concentration of optically pure L-lactic acid by alkaliphilic Bacillus sp. WL-S20, combined with a low-cost nutrient and environment-friendly NaOH-based process, represent a potentially novel way for L-lactic acid production at an industrial scale.

PMID:
22534372
DOI:
10.1016/j.biortech.2012.03.103
[Indexed for MEDLINE]

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