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Curr Genet. 1990 Nov;18(4):385-6.

Electrophoretic karyotyping without the need for generating protoplasts.

Author information

1
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2902.

Abstract

Chromosome samples for pulsed-field electrophoresis have been prepared without first generating protoplasts. The technique involves treatment of intact, agarose-solidified cell material with protease in the presence of EDTA and SDS. Saccharomyces cerevisiae, Ustilago hordei, Tilletia caries, and T. controversa karyotypes are clearly resolved with this technique. Colonies of U. hordei and S. cerevisiae removed from the surface of agar-solidified media and prepared for PFGE by this abbreviated method also yield well resolved karyotypes.

PMID:
2253276
DOI:
10.1007/bf00318221
[Indexed for MEDLINE]

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