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Osteoarthritis Cartilage. 2012 Jul;20(7):773-82. doi: 10.1016/j.joca.2012.04.014. Epub 2012 Apr 21.

Musculoskeletal changes following non-invasive knee injury using a novel mouse model of post-traumatic osteoarthritis.

Author information

1
Department of Orthopaedic Surgery, University of California-Davis Medical Center, Sacramento, CA 95817, USA. bchristiansen@ucdavis.edu

Abstract

OBJECTIVE:

Post-traumatic osteoarthritis (PTOA) is a common consequence of traumatic joint injury, with 50% of anterior cruciate ligament (ACL) rupture patients developing PTOA within 10-20 years. Currently accepted mouse models of PTOA initiate symptoms using various methods, none of which faithfully mimic clinically-relevant injury conditions. In this study we characterize a novel non-invasive mouse model of PTOA that injures the ACL with a single load of tibial compression overload. We utilize this model to determine the time course of articular cartilage and subchondral bone changes following knee injury.

DESIGN:

Mice were euthanized 1, 3, 7, 14, 28, or 56 days after non-invasive knee injury. Knees were scanned using micro-computed tomography (μCT) in order to quantify subchondral trabecular bone, subchondral bone plate, and non-native bone formation (heterotopic ossification). Development of osteoarthritis (OA) was graded using the osteoarthritis research society international (OARSI) scale on histological sections of injured and uninjured knees.

RESULTS:

Following injury we observed a rapid loss of trabecular bone in injured knees compared to uninjured knees by 7 days post-injury, followed by a partial recovery of trabecular bone to a new steady state by 28 days post-injury. We also observed considerable non-native bone formation by 56 days post-injury. Grading of histological sections revealed deterioration of articular cartilage by 56 days post-injury, consistent with development of mild OA.

CONCLUSIONS:

This study establishes a novel mouse model of PTOA, and describes the time course of musculoskeletal changes following knee injury, helping to establish the window of opportunity for preventative treatment.

PMID:
22531459
DOI:
10.1016/j.joca.2012.04.014
[Indexed for MEDLINE]
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