Format

Send to

Choose Destination
J Agric Food Chem. 2012 Jun 20;60(24):6210-6. doi: 10.1021/jf300531e. Epub 2012 Jun 1.

Mass production of rubusoside using a novel stevioside-specific β-glucosidase from Aspergillus aculeatus.

Author information

1
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.

Abstract

Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by (1)H and (13)C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.

PMID:
22530920
DOI:
10.1021/jf300531e
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center