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PLoS One. 2012;7(4):e35063. doi: 10.1371/journal.pone.0035063. Epub 2012 Apr 18.

Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.

Author information

1
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Abstract

Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism.

PMID:
22529973
PMCID:
PMC3329542
DOI:
10.1371/journal.pone.0035063
[Indexed for MEDLINE]
Free PMC Article

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