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World J Gastroenterol. 2012 Apr 14;18(14):1579-89. doi: 10.3748/wjg.v18.i14.1579.

Dual regulatory role for phosphatase and tensin homolog in specification of intestinal endocrine cell subtypes.

Author information

  • 1Canadian Institutes of Health Research Team on Digestive Epithelium, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke J1H 5N4, QC, Canada.

Abstract

AIM:

To investigate the impact of phosphatase and tensin homolog (Pten) in the specification of intestinal enteroendocrine subpopulations.

METHODS:

Using the Cre/loxP system, a mouse with conditional intestinal epithelial Pten deficiency was generated. Pten mutant mice and controls were sacrificed and small intestines collected for immunofluorescence and quantitative real-time polymerase chain reaction. Blood was collected on 16 h fasted mice by cardiac puncture. Enzyme-linked immunosorbent assay was used to measure blood circulating ghrelin, somatostatin (SST) and glucose-dependent insulinotropic peptide (GIP) levels.

RESULTS:

Results show an unexpected dual regulatory role for epithelial Pten signalling in the specification/differentiation of enteroendocrine cell subpopulations in the small intestine. Our data indicate that Pten positively regulates chromogranin A (CgA) expressing subpopulations, including cells expressing secretin, ghrelin, gastrin and cholecystokinin (CCK). In contrast, Pten negatively regulates the enteroendocrine subtype specification of non-expressing CgA cells such as GIP and SST expressing cells.

CONCLUSION:

The present results demonstrate that Pten signalling favours the enteroendocrine progenitor to specify into cells expressing CgA including those producing CCK, gastrin and ghrelin.

KEYWORDS:

Chromogranin A; Enteroendocrine cells; Intestinal epithelial cell specification; Phosphatase and tensin homolog

PMID:
22529686
PMCID:
PMC3325523
DOI:
10.3748/wjg.v18.i14.1579
[PubMed - indexed for MEDLINE]
Free PMC Article
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