Format

Send to

Choose Destination
Surgeon. 2012 Jun;10(3):123-7. doi: 10.1016/j.surge.2011.02.004. Epub 2011 Mar 25.

Raman spectroscopy--a potential new method for the intra-operative assessment of axillary lymph nodes.

Author information

1
Department of Biophotonics Research, Gloucestershire Hospitals NHS Foundation Trust, Gloucester Royal Hospital, Leadon House, Gt. Western Road, Gloucester GL1 3NN, UK. jonathanhorsnell@hotmail.com

Abstract

Sentinel Lymph Node Biopsy has become the standard surgical procedure for the sampling of axillary lymph nodes in breast cancer. Intra-operative node assessment of these nodes would allow definitive axillary surgery to take place immediately with associated benefits for patient management. Our experimental study aims to demonstrate that a Raman spectroscopy probe system could overcome many of the disadvantages of current intra-operative methods. 59 axillary lymph nodes, 43 negative and 16 positive from 58 patients undergoing breast surgery at our district general hospital were mapped using Raman micro-spectroscopy. These maps were then used to model the effect of using a Raman spectroscopic probe by selecting 5 and 10 probe points across the mapped images and evaluating the impact on disease detection. Results demonstrated sensitivities of up to 81% and specificities of up to 97% when differentiating between positive and negative lymph nodes, dependent on the number of probe points included. The results would have concurred with histopathology assessment in 89% and 91% of cases in the 5 and 10 point models respectively. Using Raman spectroscopy in this way could allow lymph node assessment within a time-frame suitable for intra-operative use.

PMID:
22525413
DOI:
10.1016/j.surge.2011.02.004
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center