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Nat Methods. 2012 Apr 22;9(7):721-3. doi: 10.1038/nmeth.1978.

Faster STORM using compressed sensing.

Author information

1
Nuclear and Radiological Engineering Program, The George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA. leizhu@gatech.edu

Abstract

In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s.

PMID:
22522657
PMCID:
PMC3477591
DOI:
10.1038/nmeth.1978
[Indexed for MEDLINE]
Free PMC Article

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