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Opt Express. 2012 Apr 9;20(8):9197-202. doi: 10.1364/OE.20.009197.

In vivo Brillouin optical microscopy of the human eye.

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1
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom St., BHX-6, Boston, Massachusetts 02114, USA.

Abstract

We report the first Brillouin measurement of the human eye in vivo. We constructed a Brillouin optical scanner safe for human use by employing continuous-wave laser light at 780 nm at a low power of 0.7 mW. With a single scan along the optic axis of the eye, the axial profile of Brillouin frequency shift was obtained with a pixel acquisition time of 0.4 s and axial resolution of about 60 μm, showing the depth-dependent biomechanical properties in the cornea and lens.

PMID:
22513631
PMCID:
PMC3500092
[Indexed for MEDLINE]
Free PMC Article
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