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Biotechnol Bioeng. 2012 Oct;109(10):2663-70. doi: 10.1002/bit.24530. Epub 2012 Apr 24.

Fluorescent silica particles for monitoring oxygen levels in three-dimensional heterogeneous cellular structures.

Author information

1
Department of Chemical, Biochemical & Environmental Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, ECS 314, Baltimore, Maryland 21250, USA.

Abstract

Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations within complex three-dimensional structures with ∼ 1 µm resolution. Such measurements may complement measures of biofilm structure and cell activity to provide a more comprehensive understanding of biofilm biology. Thus, we developed oxygen-sensing microparticles specifically designed to characterize oxygen transport through the volume of bacterial biofilms. The Stöber method was used to synthesize monodisperse silica microparticles of approximately the same size as a bacterium (∼ 1 µm). Two fluorophores, oxygen-sensitive Ru(Ph(2) phen(3))Cl(2), and the reference fluorophore Nile blue chloride were immobilized on the surface of the particles. We demonstrate application of the microparticles toward measuring the oxygen concentration profiles within a live Staphylococcus aureus biofilm.

PMID:
22511120
PMCID:
PMC3419812
DOI:
10.1002/bit.24530
[Indexed for MEDLINE]
Free PMC Article

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