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PLoS One. 2012;7(4):e34307. doi: 10.1371/journal.pone.0034307. Epub 2012 Apr 10.

Searching for cellular partners of hantaviral nonstructural protein NSs: Y2H screening of mouse cDNA library and analysis of cellular interactome.

Author information

1
Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland. samu.ronnberg@helsinki.fi

Abstract

Hantaviruses (Bunyaviridae) are negative-strand RNA viruses with a tripartite genome. The small (S) segment encodes the nucleocapsid protein and, in some hantaviruses, also the nonstructural protein (NSs). The aim of this study was to find potential cellular partners for the hantaviral NSs protein. Toward this aim, yeast two-hybrid (Y2H) screening of mouse cDNA library was performed followed by a search for potential NSs protein counterparts via analyzing a cellular interactome. The resulting interaction network was shown to form logical, clustered structures. Furthermore, several potential binding partners for the NSs protein, for instance ACBD3, were identified and, to prove the principle, interaction between NSs and ACBD3 proteins was demonstrated biochemically.

PMID:
22506017
PMCID:
PMC3323627
DOI:
10.1371/journal.pone.0034307
[Indexed for MEDLINE]
Free PMC Article

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