Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biomed Biotechnol. 2012;2012:948320. doi: 10.1155/2012/948320. Epub 2012 Mar 8.

Xenogenic esophagus scaffolds fixed with several agents: comparative in vivo study of rejection and inflammation.

Author information

1
Translational Centre for Regenerative Medicine-TRM, University of Leipzig, 04103 Leipzig, Germany. holger.koch@trm.uni-leipzig.de

Abstract

Most infants with long-gap esophageal atresia receive an esophageal replacement with tissue from stomach or colon, because the native esophagus is too short for true primary repair. Tissue-engineered esophageal conducts could present an attractive alternative. In this paper, circular decellularized porcine esophageal scaffold tissues were implanted subcutaneously into Sprague-Dawley rats. Depending on scaffold cross-linking with genipin, glutaraldehyde, and carbodiimide (untreated scaffolds : positive control; bovine pericardium : gold standard), the number of infiltrating fibroblasts, lymphocytes, macrophages, giant cells, and capillaries was determined to quantify the host response after 1, 9, and 30 days. Decellularized esophagus scaffolds were shown to maintain native matrix morphology and extracellular matrix composition. Typical inflammatory reactions were observed in all implants; however, the cellular infiltration was reduced in the genipin group. We conclude that genipin is the most efficient and best tolerated cross-linking agent to attenuate inflammation and to improve the integration of esophageal scaffolds into its surrounding tissue after implantation.

PMID:
22505820
PMCID:
PMC3312382
DOI:
10.1155/2012/948320
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Hindawi Publishing Corporation Icon for PubMed Central
    Loading ...
    Support Center