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J Biol Chem. 2012 Jun 1;287(23):19367-76. doi: 10.1074/jbc.M112.352005. Epub 2012 Apr 13.

Molecular cloning and characterization of first organic matrix protein from sclerites of red coral, Corallium rubrum.

Author information

1
Centre Scientifique de Monaco, Avenue Saint-Martin, MC-98000, Monaco, France.

Abstract

We report here for the first time the isolation and characterization of a protein from the organic matrix (OM) of the sclerites of the alcyonarian, Corallium rubrum. This protein named scleritin is one of the predominant proteins extracted from the EDTA-soluble fraction of the OM. The entire open reading frame (ORF) was obtained by comparing amino acid sequences from de novo mass spectrometry and Edman degradation with an expressed sequence tag library dataset of C. rubrum. Scleritin is a secreted basic phosphorylated protein which exhibits a short amino acid sequence of 135 amino acids and a signal peptide of 20 amino acids. From specific antibodies raised against peptide sequences of scleritin, we obtained immunolabeling of scleroblasts and OM of the sclerites which provides information on the biomineralization pathway in C. rubrum.

PMID:
22505718
PMCID:
PMC3365975
DOI:
10.1074/jbc.M112.352005
[Indexed for MEDLINE]
Free PMC Article

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