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Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):484-96. doi: 10.1107/S0907444911054515. Epub 2012 Mar 16.

PDB_REDO: constructive validation, more than just looking for errors.

Author information

1
Department of Biochemistry, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. r.joosten@nki.nl

Abstract

Developments of the PDB_REDO procedure that combine re-refinement and rebuilding within a unique decision-making framework to improve structures in the PDB are presented. PDB_REDO uses a variety of existing and custom-built software modules to choose an optimal refinement protocol (e.g. anisotropic, isotropic or overall B-factor refinement, TLS model) and to optimize the geometry versus data-refinement weights. Next, it proceeds to rebuild side chains and peptide planes before a final optimization round. PDB_REDO works fully automatically without the need for intervention by a crystallographic expert. The pipeline was tested on 12 000 PDB entries and the great majority of the test cases improved both in terms of crystallographic criteria such as R(free) and in terms of widely accepted geometric validation criteria. It is concluded that PDB_REDO is useful to update the otherwise `static' structures in the PDB to modern crystallographic standards. The publically available PDB_REDO database provides better model statistics and contributes to better refinement and validation targets.

PMID:
22505269
PMCID:
PMC3322608
DOI:
10.1107/S0907444911054515
[Indexed for MEDLINE]
Free PMC Article

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