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J Chromatogr A. 2012 Oct 12;1259:62-73. doi: 10.1016/j.chroma.2012.03.068. Epub 2012 Mar 28.

Analysis of phospolipids and glycolipids by thin-layer chromatography-matrix-assisted laser desorption and ionization mass spectrometry.

Author information

1
University of Leipzig, Medical Department, Institute of Medical Physics & Biophysics, Härtelstr. 16/18, D-04107 Leipzig, Germany. beate.fuchs@medizin.uni-leipzig.de

Abstract

Thin-layer chromatography-matrix-assisted laser desorption and ionization mass spectrometry (TLC-MALDI-MS) of organic extracts from biological samples allows untargeted analysis and structural characterization of phospholipids and glycolipids ionized from the near-surface region of a sample separated on a TLC-plate. In particular, MALDI-MS enables the sensitive detection of many analytes directly from the solid surface of an ordinary TLC-plate even without previous staining. It will be shown that the detailed fatty acyl composition of phospholipids can be determined solely by TLC-MALDI-MS without previous derivatization, enzymatic cleavage and/or reversed phase separation. MALDI-time-of-flight (TOF) MS is thus a powerful method in this field due to its high sensitivity, low extent of induced fragmentation and simple, user-friendly performance. This review summarizes the so far available knowledge about combined TLC-MALDI-MS for phospholipid and glycolipid characterization together with the technical workflow and a survey of applications. Finally a perspective on the future of TLC-MALDI-MS is given.

PMID:
22503924
DOI:
10.1016/j.chroma.2012.03.068
[Indexed for MEDLINE]

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