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J Urol. 2012 Jun;187(6):2249-53. doi: 10.1016/j.juro.2012.01.029. Epub 2012 Apr 13.

Protective effects of hydrogen rich saline solution on experimental testicular ischemia-reperfusion injury in rats.

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Department of Pediatric Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.



We examined the effectiveness of hydrogen rich saline solution on the prevention of testicular damage induced by ischemia/reperfusion in rats.


Male Sprague-Dawley® rats were divided randomly into 4 groups, including group 1-sham operated, group 2-torsion-detorsion, group 3-torsion-detorsion plus saline and group 4-torsion-detorsion plus hydrogen rich saline solution. Testicular torsion was performed by rotating the left testis 720 degrees clockwise for 4 hours. Reperfusion was allowed for 4 hours. Hydrogen rich saline solution (5 ml/kg) was injected intraperitoneally in rats in group 4 15 minutes before the start of detorsion. Rats were sacrificed after 4-hour initiation of detorsion. Left orchiectomy was done for histopathological examination and biochemical assay.


The testicular injury score in groups 2 and 3 was significantly lower than in sham operated group 1 but higher in group 4 with hydrogen rich saline than in group 2 with torsion-detorsion. The apoptosis index was significantly increased in groups 2 and 3. Hydrogen rich saline solution treatment significantly decreased the apoptosis index. A significant increase in malondialdehyde and a decrease in superoxide dismutase activity were observed in groups 2 and 3. In group 4 malondialdehyde was significantly lowered and superoxide dismutase activity was significantly improved compared with groups 2 and 3.


Results provide a biochemical and histopathological basis for the action of hydrogen rich saline solution as a therapeutic agent for testicular damage induced by ischemia/reperfusion injury.

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