Format

Send to

Choose Destination
J Proteomics. 2012 Aug 30;75(16):5036-5051. doi: 10.1016/j.jprot.2012.03.017. Epub 2012 Mar 29.

Mass spectrometry imaging and profiling of single cells.

Author information

1
Department of Chemistry and the Beckman Institute of Science and Technology, University of Illinois, Urbana IL 61801, USA.
2
Department of Chemistry and the Beckman Institute of Science and Technology, University of Illinois, Urbana IL 61801, USA. Electronic address: jsweedle@illinois.edu.

Abstract

Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enables new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling.

PMID:
22498881
PMCID:
PMC3419297
DOI:
10.1016/j.jprot.2012.03.017
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center