Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2012 May 4;421(2):208-13. doi: 10.1016/j.bbrc.2012.03.136. Epub 2012 Apr 4.

Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.

Author information

1
Centre for Structural Biology & Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ, UK.

Abstract

The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

PMID:
22497887
PMCID:
PMC3522111
DOI:
10.1016/j.bbrc.2012.03.136
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center