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J Glaucoma. 2012 Oct-Nov;21(8):530-8. doi: 10.1097/IJG.0b013e318241b83c.

Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma.

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1
Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA.

Abstract

PURPOSE:

To determine changes in the expression profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in the aqueous humor outflow pathway and optic nerve head (ONH) of human eyes with or without glaucoma to explore their potential involvement in glaucoma pathophysiology.

METHODS:

Age-matched paraffin-embedded postmortem eyes from patients with or without glaucoma were stained immunohistochemically using polyclonal antibodies raised against RhoA, ROCK-1, and ROCK-2. The intensity of the immunostaining in the aqueous humor outflow pathway and the ONH was graded by 4 individuals who were masked concerning whether the eyes were from normal individuals or those with glaucoma.

RESULTS:

Both normal eyes and those with glaucoma showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the trabecular meshwork, ciliary muscle, and ONH. There was a significant increase in the RhoA protein levels in the glaucomatous ONH compared with the age-matched controls.

CONCLUSIONS:

Elevated levels of RhoA in the ONH of glaucomatous eyes suggest possible involvement of RhoA in the pathophysiology of glaucoma.

PMID:
22495072
DOI:
10.1097/IJG.0b013e318241b83c
[Indexed for MEDLINE]
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