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Appl Environ Microbiol. 2012 Jun;78(12):4516-8. doi: 10.1128/AEM.07974-11. Epub 2012 Apr 6.

Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34.

Author information

1
Department of Proteomics and Microbiology, Interdisciplinary Center of Mass Spectrometry (CISMa), University of Mons, Mons, Belgium.

Abstract

Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α(2)β(2)γ(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.

PMID:
22492439
PMCID:
PMC3370517
DOI:
10.1128/AEM.07974-11
[Indexed for MEDLINE]
Free PMC Article

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