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Nat Methods. 2012 Apr 8;9(5):465-6. doi: 10.1038/nmeth.1973.

Derivation of iPSCs in stirred suspension bioreactors.

Author information

1
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.

Abstract

Induced pluripotent stem cells (iPSCs) are typically derived in adherent culture. Here we report fast and efficient derivation of mouse iPSCs in stirred suspension bioreactors, with and without the use of c-Myc. Suspension-reprogrammed cells expressed pluripotency markers, showed multilineage differentiation in vitro and in vivo, and contributed to the germline in chimeric mice. Suspension reprogramming has the potential to accelerate and standardize iPSC research.

PMID:
22484846
DOI:
10.1038/nmeth.1973
[Indexed for MEDLINE]

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