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Structure. 2012 Apr 4;20(4):618-27. doi: 10.1016/j.str.2012.02.018. Epub 2012 Apr 3.

How conformational dynamics of DNA polymerase select correct substrates: experiments and simulations.

Author information

1
Department of Chemistry and Biochemistry and Institute for Computational Engineering and Sciences (ICES), The University of Texas at Austin, 1 University Station, Austin, TX 78712, USA.

Abstract

Nearly every enzyme undergoes a significant change in structure after binding it's substrate. Experimental and theoretical analyses of the role of changes in HIV reverse transcriptase structure in selecting a correct substrate are presented. Atomically detailed simulations using the Milestoning method predict a rate and free energy profile of the conformational change commensurate with experimental data. A large conformational change occurring on a millisecond timescale locks the correct nucleotide at the active site but promotes release of a mismatched nucleotide. The positions along the reaction coordinate that decide the yield of the reaction are not determined by the chemical step. Rather, the initial steps of weak substrate binding and protein conformational transition significantly enrich the yield of a reaction with a correct substrate, whereas the same steps diminish the reaction probability of an incorrect substrate.

PMID:
22483109
PMCID:
PMC3322391
DOI:
10.1016/j.str.2012.02.018
[Indexed for MEDLINE]
Free PMC Article

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