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Dig Dis Sci. 2012 Sep;57(9):2296-303. doi: 10.1007/s10620-012-2156-x. Epub 2012 Apr 3.

Thymoquinone prevents and ameliorates dextran sulfate sodium-induced colitis in mice.

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1
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, People's Republic of China.

Abstract

BACKGROUND:

Thymoquinone (TQ), an active ingredient of the seed oil extract of Nigella sativa Linn, has previously been shown to possess antitumor, antioxidant, and anti-inflammatory bioactivity. Whether TQ has any effect on colitis remains controversial.

AIM:

The aim of this study was to determine whether treatment with TQ prevents and ameliorates colonic inflammation in a mouse model of inflammatory bowel disease.

METHODS:

C57BL/6 murine colitis was induced by the administration of dextran sodium sulfate (DSS) (3 % W/V) in the drinking water supplied to the mice for 7 consecutive days. The mice with colitis were treated with 5, 10, or 25 mg/kg TQ orally, and changes in body weight and macroscopic and microscopic colitis scores were examined. In addition, biochemical analyses were conducted.

RESULTS:

The treatment of mice with TQ prevented and significantly reduced the appearance of diarrhea and body weight loss. These results were associated with amelioration of colitis-related damage, as measured by macroscopic and microscopic colitis scores. In addition, there was a significant reduction in colonic myeloperoxidase activity and malondialdehyde levels and an increase in glutathione levels.

CONCLUSIONS:

These results indicate that TQ administration can prevent and improve murine DSS-induced colitis. These findings suggest that TQ could serve as a potential therapeutic agent for the treatment of patients with inflammatory bowel disease.

PMID:
22476588
DOI:
10.1007/s10620-012-2156-x
[Indexed for MEDLINE]

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