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Trends Immunol. 2012 Jul;33(7):323-32. doi: 10.1016/j.it.2012.02.010. Epub 2012 Apr 2.

A deep profiler's guide to cytometry.

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1
Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.

Abstract

In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes in individual cells. The ability to measure simultaneously multiple genes in the same cell is necessary to resolve the great diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can quantify 18 proteins per cell, at >10 000 cells/s. Mass cytometry is a new technology that promises to extend these capabilities significantly. Immunophenotyping by mass spectrometry provides the ability to measure >36 proteins at a rate of 1000 cells/s. We review these cytometric technologies, capable of high-content, high-throughput single-cell assays.

PMID:
22476049
PMCID:
PMC3383392
DOI:
10.1016/j.it.2012.02.010
[Indexed for MEDLINE]
Free PMC Article
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